FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26887497
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG35700.014.89800614
CGCGATT8850.013.4598811
CGCTCGT45000.012.28562715
CGAAGAT93750.012.1829341
TTACACG32900.012.13213532
CGCGTAT4206.194932E-811.6784271
CGTTCGC16950.011.56035316
TATATCG17250.011.36583535
TAAGTCG25500.011.11581320
GTCGTTA17750.010.84328220
CCGGGAT50050.010.7800861
CGCAGAC73750.010.6887291
CGCGGGT20050.010.3096331
ACTCGTG52300.010.30422822
ATATCGC18500.010.21905536
CGTATCG23550.010.103233
CGACAAT33600.09.9058081
CGCGAAC7859.094947E-129.8188051
CGACAAC28600.09.8000781
CGACCGT13000.09.7020771
Produced by FastQC (version 0.11.5)