FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26686742
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA72800.035.7754141
ATCGGAA83300.031.213772
ACACGTC91550.030.50917413
ACGTCTG100000.028.4108515
CACGTCT103450.027.53076214
GTCACGA58250.026.77496329
AGTCACG63300.026.10678928
TCGTATG79500.024.587343
ACGATCA65650.024.33248132
CACGATC65350.023.918531
TCGGAAG110950.023.3782623
TATGCCG85100.023.13579246
CACACGT123800.022.5615112
CGTATGC87350.022.49802844
CGATCAG74600.021.32105433
CTCGTAT90600.021.11994242
GCACACG137650.020.91539611
CGTCTGA136600.020.79810516
TCACGAT77750.020.41313730
TCTCGTA99550.018.63452141
Produced by FastQC (version 0.11.5)