FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26686742
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA28000.022.5500950
GCGTCGT34950.019.04908412
GCCGTAT35750.018.43387448
GATCGGA45600.018.0898152
CGCCGTA38450.017.31889747
ATCGGAA54050.016.028233
TATCGCG8500.014.20378722
AGCGTCG49350.013.840137511
TGGTCGC46600.013.77078243
CCGTATC48650.013.61667749
AGAGCGT50200.013.6058189
GTCGCCG49900.013.48336345
TCGCTCG39450.012.67878614
GTAGATC58450.011.39128232
AGATCGG60000.011.3316511
TCGTGTA64200.011.12235715
CGCGAAA11500.011.1003243
TCGCCGT58550.011.07894446
GTCGTGT66850.010.99094114
CGTCGTG63650.010.94746913
Produced by FastQC (version 0.11.5)