FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23282559
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG22650.022.38954443
TATGCCG27400.018.88557846
CGTATGC27400.018.75995644
CTCGTAT29700.016.7263542
AGTCACG40600.013.93438528
GATCGGA38750.013.4547761
ACACGTC43350.013.45255313
ACGGCTA50950.011.23918532
GCGGTAT33000.011.19543651
CCGGGAT52150.010.9906911
CACGTCT53950.010.93736614
CGTATCG21900.010.8712963
TTACGCG13700.010.82773154
TATCGCG8350.010.74522310
GTATCGA23450.010.7414034
TCTCGTA46650.010.42707841
CACACGT61750.09.77930912
CGAACGA26250.09.72505346
ATGCCGT58250.09.6534247
ACGTCTG60400.09.65207615
Produced by FastQC (version 0.11.5)