FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23282559
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG15750.011.82549354
GCGGTAT34400.011.5609421
TATCGCG7400.011.18625422
TACGCGA17800.010.46356955
ATCGGTA18550.010.41236218
TAGCGAC23400.09.72819526
CGCGGAT20500.09.6155651
GTATCGA22250.09.4559254
CCGACAT39850.09.1988041
CGTATCG22300.09.1253883
CCAATCG24250.09.10274615
CGACCTA9601.8189894E-128.9819984
GCGCGAT20050.08.9689741
TAAACGC34800.08.82096152
CGGGCAT65900.08.7636471
TTACACG23650.08.75038432
CGTATCA17800.08.71960450
GTTACGC22650.08.52756353
CCGGGAT56200.08.36866951
CCCGTAT24450.08.3450131
Produced by FastQC (version 0.11.5)