FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31961156
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA86600.034.1460461
ATCGGAA99300.029.8354432
ACACGTC119150.028.4612813
ACGTCTG134000.025.4086915
CACGTCT139300.024.44199614
TCGGAAG130650.022.8846473
CACACGT159650.021.3265312
GCACACG171800.020.45230511
GTCACGA61150.019.4317229
CGTCTGA179450.019.14400316
AGTCACG66100.018.85219828
CGGAAGA192050.017.9411354
TCGTATG98800.016.88579243
TATGCCG102900.016.51351546
ACGATCA71250.016.39047632
CACGATC71050.016.34077531
CGTATGC106000.015.90137444
AATCTCG77900.015.865664541
AGCACAC229000.015.56671410
AGAGCAC233100.015.424328
Produced by FastQC (version 0.11.5)