FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31961156
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA36550.025.38871250
GCGTCGT40400.024.67606512
GCCGTAT41050.023.19324348
GATCGGA49550.022.1499862
CGCCGTA46300.020.11605847
ATCGGAA59850.019.2030143
AGAGCGT62750.016.7115969
AGCGTCG60750.016.5236311
CCGTATC60050.015.79760949
GTCGCCG61650.015.779345
TGGTCGC56800.015.60896743
CGTGTAG74950.014.40402616
GTCGTGT75300.014.24577514
TCGTGTA77700.014.02740215
TCGCCGT68650.013.9690846
TCGCTCG50400.013.55050114
GTAGATC71950.013.42664932
AGATCGG74200.013.39642051
GGTCGCC72650.013.29490744
CGTCGTG79000.013.14288413
Produced by FastQC (version 0.11.5)