FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19264377
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA249180.12934755170125667TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA249150.12933197891631792TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG92100.032.543851
TCGTATG115750.030.16163845
TATGCCG117100.029.99441548
CGTATGC117800.029.90404746
AGTCACG127500.028.16662628
TATCTCG124000.028.04319441
CTCGTAT124400.027.7591244
ACACGTC134050.027.15514213
TCTCGTA128500.026.76582743
GTCACGT133450.026.67813129
GATCGGA138850.026.566111
ACGTCTG139000.026.2345515
ATGCCGT134150.025.97647349
CACGTCT144650.025.3798814
ATCGGAA146700.025.1401462
GCCGTCT141750.024.9487151
ATCTCGT139650.024.67818542
ACGTGGC146050.024.58914632
TCACGTG146250.024.34322530
TGCCGTC148000.023.98850450
Produced by FastQC (version 0.11.5)