FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19264377
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG76450.036.83386645
CGTATCA76100.036.7766250
GCCGTAT77700.036.45960648
CGCCGTA78900.035.9953347
GCGTCGT84500.033.93985412
TCGCCGT80900.033.74379346
AGATCGG87400.033.6739921
TGGTCGC83900.033.36857643
CCGTATC86450.032.7313349
GGTCGCC91450.030.83963644
AGCGTCG96850.030.11324311
CGGTGGT95650.029.46495240
CGTCGTG100250.028.91898513
TCTCGGT102000.028.64531937
AGAGCGT105200.027.9180159
GTAGATC105300.027.45345732
GATCGGA108100.027.3532242
TCGGTGG108250.027.17143839
CGTGTAG108050.026.80901516
GTATCAT107700.026.40159851
Produced by FastQC (version 0.11.5)