FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31870246
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGA82150.012.09888611
CGCGAAA14900.011.97984743
CGTATCG30050.011.2969493
CGACCTA19150.011.147954
AGCATCG91900.011.119919
GCATCGG94800.010.55825810
CCCGTAT40050.010.5064411
TCGCTCG46850.010.15951222
ATCGGAG98950.09.76175512
TACCGTA23950.09.6443379
CTCGACT29650.09.5793761
CGACTAA32150.09.47048528
ACCGTAT24500.09.42784610
CGCGTTC20800.09.42235214
CGCGCTA15250.09.41014546
TTACACG37800.09.35119132
CGTTCGC19600.09.28516
CCGTATC44950.09.2651222
CGCGTAA7201.6989361E-99.2533281
CGACCGT13700.09.2142261
Produced by FastQC (version 0.11.5)