FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31870246
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGA78000.012.06965211
CGACCTA19000.011.9714784
CGCGAAA13950.011.79242143
AGCATCG88400.010.8867719
GCATCGG88550.010.7896110
TCGCTCG46400.010.55975722
CGTATCG29300.010.3904633
CCCGTAT39850.010.2739161
CTCGGAT69600.010.1559511
GTATCGA35200.010.0407634
TAAGTCG32100.09.92180120
ATCGGAG95850.09.74892912
TACCGTA21350.08.8514539
TACCGGT15150.08.77903744
CGCTCGT56850.08.74173523
CGACCGT14250.08.5947191
CTCGGGT66000.08.5891371
CGACTTA33150.08.44703367
CCGGCAT48950.08.4354411
TCGGGTC50400.08.4010322
Produced by FastQC (version 0.11.5)