FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30986214
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG32050.012.80931814
CGACCGT11200.012.3443131
CGAAGAT91700.011.0816131
CGATAAC24700.011.0549071
TAAGCGA23650.010.94034146
GTCGTTA19650.010.885035520
CGCGGAT19450.010.3070261
CCGGGAT57450.09.9871831
CGCAGAC79600.09.8134181
CGAGAAT56750.09.8058431
CGCTCGT42900.09.73049815
CTCGGTT33150.09.696711
CGCGTAT5059.433297E-79.582121
CGCGGGT24200.09.5693931
CGTTCGC18750.09.56768916
CGCCAAC45400.09.4404081
CGCGGTT15900.09.1301331
CTCGGGT64750.09.12811851
CCGGCAT51300.09.0958091
CGGGGAT55900.08.9038051
Produced by FastQC (version 0.11.5)