FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30986214
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12800.014.82298851
TAAGCGA21600.012.93367146
TCGCTCG38750.012.82129914
CGCAGAC79050.011.2590281
CGTTCGC17800.010.85448616
CGAAGAT91150.010.67272951
CGCGCAT9800.010.56034851
CGCTCGT48850.010.52356615
CGCCAAT51200.010.1739611
ACTCCGC36850.09.9221525
CGGGGAT54000.09.6464221
TCGGGTC51250.09.4231522
CCGGGAT57250.09.4000941
CGACCTA14050.09.3294044
CGTATCG19100.09.2108313
CTCGGAT66650.09.1612811
GCTCGTA57650.09.09673416
CGCAAAT31950.09.0696711
CCGGCAT48400.09.0519351
CGCGGGT20600.09.0429391
Produced by FastQC (version 0.11.5)