FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30263338
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG28600.023.8838943
TATGCCG34200.020.47819146
CGTATGC35050.019.6862844
CTCGTAT41350.015.60170342
ACACGTC50850.014.58884413
GTATCGA32750.014.2217644
GATCGGA50400.013.6963741
TTACGCG22750.013.194139554
GCGGTAT55100.012.5280811
CGTATCG32600.012.4879073
CCCGTAT35250.012.1413991
TACGCGA25000.011.8686655
CACGTCT65300.011.6247314
CCGTATC45600.010.7435732
ATCGAAG73900.010.6915686
CACGATC59500.010.55164436
TCACGAT68400.010.38905635
GTCGCTA25000.010.21059327
ACGTCTG76900.09.91401615
CGAGCGA54300.09.84835353
Produced by FastQC (version 0.11.5)