FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30263338
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA28500.016.0089634
CGTATCG32150.015.46233753
CCCGTAT36450.014.2237291
TTACGCG22100.013.09582454
GCGGTAT51400.012.2101821
TACGCGA25600.011.4384655
CCGTATC54500.011.432942
ATCGAAG67250.011.1386996
TATCGAA41750.010.3573955
CCGACAT54900.010.2513021
CGCGGAT26650.010.2390621
CGCGTAA30650.09.7930871
GTTACGC32150.09.63751753
GCGCGAT24850.09.6081341
ACGCGAC30550.09.58509256
TCGAAGG85200.09.4713697
CGCGAGT23300.09.2225721
CGACCTA19500.09.0797114
GTCGCTA28250.09.039535527
AATCGGG48250.09.0326665
Produced by FastQC (version 0.11.5)