FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35255435
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG53500.015.47806514
CGCTCGT63600.012.96574515
CGCAGAC89250.012.13581851
TACTCGT76750.010.96400421
CGAAGAT117700.010.9315061
CCCGATA76350.010.75761169
ACTCGTG78250.010.75383122
CTCGTAC79650.010.569653517
CCGGGAT54350.010.4720581
TTACACG43800.010.4041132
TCGTGCG79500.010.36803624
CGCCTCG82800.010.33403729
GCTCGTA83300.010.06509716
CGCAACC87050.09.947124550
ACCCGAT82250.09.89823268
TAAGCGA26750.09.798246
CGTGCGC86300.09.71115925
TAAGTCG32900.09.64396920
CGGGGAT64750.09.6424291
CGTATCG24050.09.4658023
Produced by FastQC (version 0.11.5)