FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24574295
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA34350.036.3542912
CATACGA30900.035.38749715
GCATACG36000.034.305614
ATACGAG37300.028.483416
AGACGGC79950.022.5233049
TATCGCG6600.022.47552722
AAGACGG80950.022.2452018
ACGGCAT67250.020.0054711
TACGAGA46350.018.15988317
GACGGCA88000.017.7188610
GAAGACG107550.017.1921427
CTCGGGT55550.015.8981481
CGTATCA25500.015.42214250
TTACACG28000.015.40290932
ACGAGAT51900.015.28742318
GCCGTAT27500.014.92905648
TCGGGTC46100.014.8919842
GCGTCGT29250.014.62411612
CTCGGTT32450.013.9266691
GGCATAC94950.013.478765513
Produced by FastQC (version 0.11.5)