FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30403539
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG43500.030.1854943
TATGCCG48250.027.42656146
CGTATGC50150.026.18282944
CTCGTAT59150.022.66125942
GATCGGA61550.021.8084091
ACACGTC65700.020.5076413
TATCTCG63650.020.25345839
ACCGATG67150.020.12195832
CACCGAT78450.017.22354731
CGATGTA78550.017.07063734
CACGTCT82200.016.68235814
TCTCGTA80800.016.1660841
ATGCCGT85700.015.52124247
CCGATGT87700.015.05620333
ACGTCTG92650.014.76384315
CACACGT93900.014.34879612
TCGGAAG96350.014.12583353
CGTATCG30700.013.81233
CCCGTAT32500.013.3809311
GCGGTAT62750.013.3695971
Produced by FastQC (version 0.11.5)