FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41373326
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57400.015.61044146
TCGTATG57900.015.59652143
CGTATGC62700.014.51435144
CTCGTAT72100.013.25296142
TCGCTCG63550.012.9963414
TATCTCG70850.012.7455839
ACACGTC80200.011.694591513
ACCGATG81950.011.61639532
CGATGTA79850.011.439934
CCGGGAT80100.011.2487371
GATCGGA74300.010.9943681
TCTCGTA83450.010.77934441
CTCGGGT83600.010.7777971
CGCTCGT77750.010.71275615
TCGGGTC63650.010.6116662
ACGTCTG92800.010.408442515
CGAAGAT149600.010.3349911
CGCGGGT27450.09.9622041
CACCGAT97150.09.94298231
CGCAGAC117000.09.82858851
Produced by FastQC (version 0.11.5)