FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41373326
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG68600.012.70358214
TTACACG48600.012.24142732
CGTATCA36100.012.21808150
CGTATCG33450.011.7168943
CGTTCGC26600.011.57865916
CGAAGAT146900.011.3628341
TCGGGTC66300.010.8728332
CGCAGAC118850.010.6291391
GTATCGA46450.010.5469814
CTCGGGT87550.010.4321641
GCCGTAT42500.010.37804448
CGCTCGT85100.010.32289315
CGCGTTC31200.010.320106514
CCGGGAT82250.09.9131141
CGACCGT20100.09.5753951
CGCGGGT29550.09.4737631
CCCGTAT51250.09.4226981
CCGCGTT36800.09.12943513
CTCGGAT95800.09.0954441
CTCGTAC95900.09.08753117
Produced by FastQC (version 0.11.5)