FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23170971
Sequences flagged as poor quality0
Sequence length75
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT18600.030.49334735
CCGTACG23450.024.74915534
ACCGTAC25850.023.08918233
TACGTAA25250.022.85401537
TCGTATG42950.021.57871446
GTACGTA27400.021.06086236
CGTATGC45900.020.69484547
TATGCCG46000.020.4350249
GATCGGA47900.019.6216492
ACACGTC54150.018.99887714
CGTAATC28600.018.79304139
AGATCGG50050.018.651931
ACGTAAT30500.017.94662938
CTCGTAT56250.016.76903745
ATCGGAA56850.016.474463
ACGTCTG64400.016.0261216
TTACACG26650.015.59063932
CACGTCT66350.015.35633115
TCTCGTA60800.015.24259644
AATCTCG39750.014.59984642
Produced by FastQC (version 0.11.5)