FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23170971
Sequences flagged as poor quality0
Sequence length75
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT31200.031.84823612
GCCGTAT29750.029.70287748
CGTATCA31250.028.06524550
GATCGGA40350.025.4570352
AGCGTCG41300.024.46331611
CGCCGTA36500.024.30123747
AGAGCGT45600.022.375849
ATCGGAA48200.021.310933
CCGTATC43450.020.72055649
CGTGTAG48800.020.62812416
GTCGTGT49350.020.39787314
GTCGCCG44300.020.24683845
TGGTCGC43100.019.65096543
TCGTGTA51650.019.42463315
AGATCGG51450.019.3811231
TCGCCGT45250.018.86448946
CGTCGTG54900.018.46308313
GAGCGTC59250.017.50228510
AAGAGCG62700.016.8585078
GTAGATC56250.016.65604232
Produced by FastQC (version 0.11.5)