FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18817594
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA368180.19565731942138828TruSeq Adapter, Index 22 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA302190.16058907424615496TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG114300.040.7000851
TCGTATG157300.040.36437645
CGTATGC172650.036.91637446
CTCGTAT168650.036.75951444
AATCTCG169200.036.52036341
TCTCGTA170100.035.80341343
CGTACGT182000.035.42161634
ATCTCGT173150.035.21951742
TACGTAA184800.034.90393436
CCGTACG188600.034.77573833
TATGCCG187350.034.73610348
GTACGTA186700.034.60260835
ACCGTAC191650.034.222332
ACGTAAT187900.033.8766437
CGTAATC184150.033.7926238
ACACGTC202650.032.74165313
GATCGGA206850.032.534621
ATGCCGT203250.031.95196549
CACCGTA205800.031.885831
ATCGGAA213500.031.5862562
Produced by FastQC (version 0.11.5)