FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18817594
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG113100.040.7480431
CGTATCA104300.040.17305450
GTCGCCG111150.039.06775345
ACGTACG15300.038.7768926
CGCCGTA112050.038.6546747
TGGTCGC112300.038.4036543
GCCGTAT111000.038.120348
GCGTCGT116800.038.0215412
TCGCCGT115650.035.81440746
CCGTATC117900.035.8018349
CGTACGG17300.034.8920727
GGTCGCC126900.034.66360544
CGGCATA27300.034.58657512
TCTCGGT133000.034.14031637
AGCGTCG132200.034.00855311
CGGTGGT127900.033.8996440
CGTCGTG132200.033.59258713
TCGGTGG136400.033.40685339
AGAGCGT136950.033.0809949
GTAGATC133800.032.6989732
Produced by FastQC (version 0.11.5)