FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43020674
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG55500.013.05076714
CGGGACG52450.011.407742510
CCGGGAT90950.011.1694231
CGCTCGT66150.011.0025215
TTACACG43750.010.88386332
TATATCG27000.010.76266735
CGCGAAT16350.010.4981741
CGCAGAC107400.010.4045141
ATATCGC30050.010.0194536
CGAAGAT133900.09.9411911
CTCGGTT53700.09.784811
CTCGGAT98600.09.7699241
CGCGTAT7554.0017767E-119.7433381
CGGGAAT75600.09.730451
CGCGAAA17350.09.68336143
ACTCGTG82500.09.37457322
CGTTCGC25650.09.139997516
CGCCTCG87100.08.922173529
CGCGTTC29250.08.8524714
CGAGAAT83400.08.7364031
Produced by FastQC (version 0.11.5)