FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43020674
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG59000.012.813292514
CGCTCGT68000.011.37477715
CGAAGAT138950.010.8814371
CGCAGAC109950.010.2181161
CGGGACG54200.010.13814210
CTCGGTT55850.010.0266961
TTACACG44600.09.96612632
CTCGTTT69850.09.8208871
CTCGGAT97900.09.7598011
CGCGGGT38550.09.7144991
CGCGATT12000.09.6248451
TATATCG27550.09.527935
CGATAAC38500.09.3634841
ACTCGTG82250.09.31896622
CGTATCG35700.09.3135753
ACCCGAT85600.09.160604568
GTATCGA41700.09.0646024
CCCGTAT51100.08.9724581
CGATCCA62400.08.9181254
TCGGGTC70450.08.8926882
Produced by FastQC (version 0.11.5)