FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29389052
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG32250.014.2358143
GTATCGA30100.013.6465324
CCCGTAT32700.013.6176551
CGCGTAA18250.013.240231
TTACGCG17250.012.791254
TTACACG31550.012.13682332
CCGTATC48400.012.1245032
ATCGAAG59200.011.6608696
CGAGCGA45900.011.41991653
GCGGTAT48050.011.2788971
TACGCGA20600.010.87786455
TCGATTG32700.010.87004720
GCGGAAT37200.010.8568361
TCGGGTC63450.010.7175362
CCGGTAT36800.010.5996371
CGCGCGT28700.010.5842751
GCGCGAT27700.010.4679071
AATCGGG52200.010.4475485
GTCGCTA18200.010.43081727
TTAGACG26200.010.40114917
Produced by FastQC (version 0.11.5)