FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30019566
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG36600.022.6212943
CGTATGC42100.019.66601444
TATGCCG44450.018.54869346
CTCGTAT47500.017.35767442
TATCTCG48000.016.74564639
GCGTCGA24100.015.60297811
GTCGCTA61950.015.09090127
GATCGGA53150.014.5699021
ACCGATG59600.014.3546532
ACACGTC57350.014.13619413
CCGACAT77850.013.9882641
CGATGTA59500.013.97292234
TCGCTAT68450.013.85946628
CGTCGAT28800.013.29622912
ATCGAAG90850.013.2528316
CACCGAT66650.012.836266531
TTACGCG34600.012.66346254
CGGTCTA50750.012.37150544
CCGATGT68600.012.27023533
GAACGCT78650.012.10591335
Produced by FastQC (version 0.11.5)