FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30019566
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGA27300.017.81986611
CGTCGAT31300.015.65381512
GTCGCTA63850.013.83112727
GCGTAGC38600.013.76312310
CCGACAT77900.013.5041451
CGTGCGT42100.013.2729888
ATCGAAG86200.013.163316
TCGCTAT69900.012.68418928
CGTATCA27500.012.67088650
AGTGTCG39150.012.516870551
GTGCGTT39500.012.48868541
CGACATC83800.012.3025082
CGGTCTA48300.012.20903444
AGTGCGT43700.012.0046840
GAACGCT76050.011.84858935
CGTTCGA42050.011.56339744
GTGTCGA44300.011.29578752
CGCTATG78850.011.24536629
CATCGAA94250.011.1943625
GACATCG94950.011.1483323
Produced by FastQC (version 0.11.5)