FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16628866
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA45250.052.37392815
CGGCATA52000.051.1364712
GCATACG53550.049.97865714
ATACGAG48400.046.03267316
ACGGCAT75650.034.42459511
TACGAGA57600.034.26186817
ACGAGAT57800.033.7360118
AAGACGG84350.032.4479568
AGACGGC86250.032.407029
GGCATAC88750.030.57781813
GACGGCA90100.029.35765310
CGAGATC72350.026.81056419
GATCGTA8450.026.71874222
GAAGACG105750.026.3138927
ATACGCG3800.025.91923116
CGTATCA37550.024.85547650
AGATCGT41000.023.76845421
GATCGTG33800.023.43749422
GAGATCG93150.020.4232120
GCCGTAT55700.019.7445868
Produced by FastQC (version 0.11.5)