FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29390317
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG65450.028.71639443
TATGCCG68600.027.50066846
CGTATGC70850.026.33091444
CTCGTAT76300.024.54108642
GATCGGA73900.024.529791
ACACGTC81850.023.21773313
ACGTCTG88350.021.66803415
ATCGGAA84550.021.5656262
ATCTCGT91300.020.24028440
CACGTCT95700.019.93073314
ACTTAGG97200.019.69530332
ATGCCGT97450.019.46673247
TCTCGTA95800.019.21670341
CACTTAG99750.019.15672931
TTAGGCA108750.017.82886134
CTTAGGC112250.017.24171333
CACACGT117900.016.32629212
TCACTTA114500.016.32209430
CCGTCTT115450.016.31018350
TCGGAAG114600.016.032923
Produced by FastQC (version 0.11.5)