FastQCFastQC Report
Sat 4 May 2024
EGAF00004859450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39609079
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4637531.1708250020153208Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2475650.6250208443372288Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGCAGAAGACGGCATACGAGATTCTGACATGTGACTGGAGTTCAGACG411370.10385750196312314RNA PCR Primer, Index 40 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)