FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39609079
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAA532070.1343303135122127Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA570200.063.03754415
CGGCATA635450.062.65070712
GCATACG632550.062.27240414
CGGCGTG554500.060.08170312
AGACGGC895700.059.997759
ATACGAG526800.059.91399816
AAGACGG899350.059.716338
ACGGCAT733850.059.43856411
ACGGCGT241700.058.96165511
GGCATAC698250.056.754113
GACGGCA757850.056.6482510
GAAGACG941700.056.343697
GGCGCCG542950.055.12948244
CTCGGGG891950.055.0434437
TCGGGGG1061550.054.99530438
AGCGGCG409100.054.3673210
TACGAGA379350.053.52829417
GCGGCGT414900.053.51354611
CGAGATT365250.053.045219
ACTCGGG336000.052.1567736
Produced by FastQC (version 0.11.5)