FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24295979
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG48050.034.89380343
TATGCCG49800.033.52906446
CGTATGC52350.031.89584444
CTCGTAT58900.028.11456342
GATCGGA62100.024.7058541
TCTCGTA68850.023.85105141
ACACGTC71500.023.54606213
ATCTCGT75250.021.8219440
ACGTCTG79350.021.1731615
ATCGGAA72650.020.8946022
ATGCCGT82700.020.77443747
CACGTCT84350.020.04082114
CACACGT93500.018.1912
GCCGTCT101850.016.8001349
CCGTCTT102500.016.76090650
GATCTCG98650.016.71566639
CGTCTGA104800.016.36061516
TCGGAAG95150.016.3161983
GTATGCC104200.016.28931245
GCACACG111050.015.96768511
Produced by FastQC (version 0.11.5)