FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24295979
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT49700.010.3532041
CGAAGAT76600.010.0085021
TTACGCG4159.551495E-69.97463454
CGCGATT9350.09.6029931
CGTATCA22650.09.44248749
CGCGTAA5251.6196063E-69.2090241
CCGGCAT50950.08.8791631
TCGCTCG39650.08.87519614
GCCGTAT27300.08.8450147
GATCGGA47450.08.8063121
CTCGGTT31800.08.6877581
TAAGCGA14350.08.65393246
TATCGCG6101.3522586E-68.4867720
GTATCGA20350.08.4775684
CGCAGAT50600.08.3263421
CGCAGAC75200.08.3120011
CCGCAAT32950.08.2797371
CGCCAAT44450.08.23529151
CTCGTAT22800.08.1790681
CTCGGGT59650.08.163071
Produced by FastQC (version 0.11.5)