FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21041436
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG60250.042.6002643
TATGCCG64750.039.10674746
CGTATGC66150.038.59208744
CTCGTAT66950.037.97635742
ACACGTC74550.036.37182213
GATCGGA64100.034.053071
ACGTCTG84800.032.0568315
CACGTCT86500.031.54645714
TCTCGTA80600.031.54472741
ATGCCGT85800.029.1507847
CACACGT93850.028.92881612
ATCTCGT88700.028.66416240
AATCTCG93850.026.90736439
GCCGTCT94650.026.20661249
GCACACG107700.025.59300411
GACCAAT100950.025.46013835
CGTCTGA107400.025.43964816
ATCGGAA87300.025.1751562
ACCAATC101900.024.8503636
TCGGAAG90450.024.7174763
Produced by FastQC (version 0.11.5)