FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21041436
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA22750.039.7229315
GCATACG28150.037.2043414
CGGCATA29350.034.56329712
ATACGAG29400.028.59361816
AGACGGC65550.020.472129
ACGGCAT54250.019.78743411
AAGACGG79550.017.6621328
GGCATAC69400.015.96897613
ACGAGAT43750.015.79659318
GACGGCA74700.015.77129110
TACGAGA43500.015.5459717
CGAGATT48350.014.36583719
GCGGTAT21700.012.7381571
CGTATCA17000.012.70054950
GAAGACG111450.012.6952957
TTACGCG10700.010.88987354
CAAGCAG193000.010.3789281
CCCGTAT20600.010.2815921
CGCGTAA16100.010.2566981
ATACGCG3853.8676237E-510.00776716
Produced by FastQC (version 0.11.5)