FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30399665
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG44500.023.6533245
CGTTTCG21950.022.62776233
TATGCCG48150.022.29263748
CGTATGC50300.020.9267646
CTCGTAT57050.018.26654444
ACACGTC64700.017.8593213
AGTCACG50100.015.97227428
ACGTCTG74500.015.833528515
GATCGGA61450.015.3490481
CGTACGT11600.015.16457134
TCTCGTA69850.015.06098943
CACGTCT81400.014.32212214
AATCTCG47150.014.26506341
ATCGGAA69150.013.6392262
ATCTCGT83050.012.99946542
TTACACG35300.012.60462932
TCGCTCG46000.012.52214
GTCACGT59800.012.45873829
CGTATCG19400.012.1095063
ATGCCGT89000.011.98312549
Produced by FastQC (version 0.11.5)