FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27155328
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG52400.021.08792945
TATGCCG54200.020.63925248
CGTATGC57100.019.77020846
TATCTCG65900.016.97492441
ACACGTC68750.016.96686413
TATCGCG8250.016.535920
CTCGTAT68100.016.52673344
TTACACG31950.016.22772632
GATCGGA64700.015.3996731
TCTCGTA73750.015.07564143
CGCGAAA10900.014.39300543
ACGTCTG81200.014.28140915
CACGTCT85000.014.12446214
TCGCTCG44700.013.58190314
ATCTCGT84450.013.286653542
ATCGGAA75900.013.0767372
ATGCCGT87100.012.60831649
ATATATC86400.012.18678738
CGAAGAT88650.012.0090541
GATATAT93950.011.61699337
Produced by FastQC (version 0.11.5)