FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27155328
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA30350.020.6875150
GCCGTAT33300.018.95745348
TTACACG32250.017.11222832
CGCCGTA39900.016.16671647
GCGTCGT41700.015.48918312
CGTATCG17200.014.845993
GATCGGA50650.014.1023412
TATCGCG7400.013.98335622
CCGTATC47400.013.68280149
CGAAGAT93450.012.8133211
AGCGTCG53850.012.63645211
CTCGGTT35400.012.4772311
CGCGAAA11250.012.26380943
GTCGCCG52650.012.0544645
TCGCCGT51950.011.88532446
TCGCTCG45150.011.84118314
CTCGGAT68950.011.811071
ATCGGAA60750.011.7011053
TAAGTCG42250.011.59301420
TGGTCGC53250.011.52970843
Produced by FastQC (version 0.11.5)