FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32827154
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACG36900.012.33026532
CGCGAAA14000.010.50035143
CGTATCG32650.010.0756323
CCCGTAT36800.09.9077681
CTCGGTT42900.09.5613441
TACCGTA22650.09.4251739
GTATCGA38750.09.3023614
CCGGGAT61100.09.2953161
CGAAGAT116850.09.2108561
CTCGTTT50250.08.8604961
CTCGGGT76200.08.6955531
ACCGTAT22150.08.68993710
CTCGGAT80750.08.4226661
CGACGAT29300.08.3756941
TACACGA57750.08.302814533
CGCGATA4653.0516958E-48.27882
ACGAAAG63450.08.1638736
TCGGGTC62250.08.1518522
CTCGACT38800.08.13207151
ATCGCGC36350.07.99189367
Produced by FastQC (version 0.11.5)