FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32827154
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG33250.012.9452833
CCCGTAT41300.012.2014061
GTATCGA40250.012.1717844
TTACACG37200.011.759732
CGCGAAA13450.010.14856343
CGCGTAA6704.783942E-109.92375851
CTCGGTT43050.09.7545111
CTCGGAT82450.09.7194611
CGAAGAT115300.09.6211591
ATCGCGC37100.09.52936667
CTCGGGT72850.09.511151
CTCGTAT34550.09.2170251
CCGTATC53950.09.0810072
CTCGTTT50650.08.9817571
CTCGAAT60050.08.8578431
CCGGGAT60600.08.777451
ACGAAAG59050.08.65322536
TCGGGTC57950.08.5145772
CGCGAAT15650.08.497021
CGTGTTA41750.08.3846859
Produced by FastQC (version 0.11.5)