FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32822820
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG51100.018.82670845
TATGCCG51550.018.33113548
CGTATGC56350.016.83101546
TATCTCG60500.015.56292741
CTCGTAT64950.014.86460844
GATCGGA74350.013.19421
TCTCGTA78650.012.83923543
ATCTCGT80400.012.26275342
TATCGCG7650.012.04075720
ACACGTC79750.011.89095213
ATCGGAA86100.011.359582
ATGCCGT88900.011.20500549
ACGTCTG89350.011.1480815
ATATATC98550.010.62661338
CCGGGAT64100.010.0394491
CACGTCT100000.09.99492214
CGAAGAT97550.09.8551421
GATATAT105350.09.81138737
CGGGGAT65800.09.780071
CGCGGAT26850.09.6454761
Produced by FastQC (version 0.11.5)