FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27011512
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG36700.014.11003214
CGCTCGT44600.011.92452415
CGCGGGT23600.011.7302031
CGCAGAC74150.011.2475061
CCGGGAT46000.011.0458881
TATGCCG44000.010.7352623
CGTATGC47400.010.128211
CTCGGAT60600.09.8881251
CTCGTTT41500.09.8793471
GTCGTTA16900.09.7331220
CGCGAAA10500.09.66489943
ACTCGTG53250.09.66324622
CTCGTAC54250.09.6097417
CGAAGAT88650.09.6054561
CGACCGT11900.09.4230931
TCGTATG36300.09.35090743
TCGTGCG55750.09.29449624
CCCGATA56300.09.26236669
CGACGAT21750.09.18345451
CGGAGAT103500.09.1752821
Produced by FastQC (version 0.11.5)