FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26410795
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG60850.035.65955433
TCGTATG66000.033.0356545
TATGCCG67200.032.54747848
CGTATGC67900.032.06042546
CTCGTAT74350.028.58310344
AGTCACG84100.026.50192828
AATCTCG81050.026.30541641
GTTTCGG85100.025.66019634
TCTCGTA82850.025.65062143
ACACGTC87450.025.48395213
ACGTTTC89050.024.32810432
TTTCGGA89850.024.03488735
GATCGGA79350.024.0259531
GTCACGT93100.023.64256929
TCGGAAT93300.023.4049637
TTCGGAA93600.023.40365836
CGGAATC93750.022.8510538
ATCGGAA86500.022.1765442
ATCTCGT95750.022.08672742
CACGTTT100250.021.71338531
Produced by FastQC (version 0.11.5)