FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26410795
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA36250.017.98620849
GCCGTAT41500.016.29272747
CGCCGTA48050.013.85635246
GATCGGA65450.012.9819121
CCGTATC53800.012.50368948
TCGCTCG40750.012.3624814
GCGTCGT53950.012.34236911
AGCGTCG67400.012.13154710
ATCGGAA70750.011.9973162
AGAGCGT73900.011.0178118
CCGGGAT56550.010.6274681
GTAGATC68150.010.57986631
CGAAGAT83450.010.4714621
CGCAGAC82750.010.2261291
ACCCGAT50250.010.16064668
CCCGATA49100.09.90649769
GTATCAT68300.09.89968150
CGCGATT10550.09.4942071
TTACGCG5101.1076754E-69.46986254
AAGAGCG92800.09.4051687
Produced by FastQC (version 0.11.5)