FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36437812
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG55900.024.19134743
TATGCCG60150.022.48206546
CGTATGC63150.021.4686644
CTCGTAT70750.018.7236542
ACACGTC84400.016.06389213
GATCGGA82900.015.3469781
TCTCGTA90350.014.73821541
ATCTCGT93300.014.08734340
ACGTCTG99300.013.6882415
ATGCCGT104200.013.67313547
TCGCTCG43450.013.49770114
ATCGGAA97400.012.9689252
GCCGTCT109800.012.47327449
CACGTCT110100.012.31419114
CACACGT117200.011.98029312
CGCTCGT51000.011.634815
GATCTCG116800.011.54834439
GCACACG127050.011.45878111
CCGTCTT119850.011.34097850
CGTATCG26050.011.2587743
Produced by FastQC (version 0.11.5)