FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36437812
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA32900.018.14402812
CATACGA26550.017.67345615
GCATACG33700.016.78982414
TCGCTCG44650.013.44497514
ATACGAG36000.011.88403416
CGCTCGT53450.011.48998415
CGTATCG29400.010.789283
CGCAGAC86250.010.4772231
CGAAGAT109000.010.1573991
CCGGGAT62350.010.0125831
CTCGGAT78000.09.8166281
GTATCGA36850.09.45008854
ACTCGTG65100.09.37661722
TCGTGCG63000.09.361649524
AGACGGC78300.09.2511459
CTCGGGT68500.09.2143461
CGTATCA39650.08.9620750
CCGGCAT55200.08.9351321
CTCGTAC69050.08.89400717
CGACGAT26850.08.8635721
Produced by FastQC (version 0.11.5)