FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51838616
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG53450.013.29870754
GCGGTAT91900.013.25180151
TACGCGA57900.012.515017555
GTATCGA44500.011.3946824
ATCGAAG116700.011.32196
CGCGAAT18450.011.0324341
CCCGTAT53550.010.6301691
GTTACGC71500.010.42415853
CCGACAT99150.010.2994661
ATCGGTA43000.09.948318518
CGTATCG60100.09.7565043
GTCGCTA75050.09.7010527
ACGCGAC73950.09.56546356
TCGACCG64800.09.47821431
TAAACGC99600.09.31936352
CGAGCGA92750.09.263576553
TAACGCG17150.09.2543433
CGCGGAT43400.09.2211271
CCGATAA83800.09.17866120
CGGTCTA44850.09.15320144
Produced by FastQC (version 0.11.5)