FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39547108
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG84850.021.36347245
TATGCCG93050.019.36836648
CTCGTAT98200.018.13861344
CGTATGC101200.017.94815346
GATCGGA103650.017.0378191
ACACGTC118850.016.95753913
AATCTCG109800.016.89153341
TCTCGTA112100.015.88948843
ACGTCTG139650.014.75753815
CACGTCT141750.014.39080214
ATCGGAA122900.014.377412
ATCTCGT130450.013.6813142
ATGCCGT143050.012.745357549
CACACGT163850.012.449779512
TCGCTCG42700.012.04561514
CGCGAAA16100.011.7372343
GCCGTCT159100.011.70158651
CCGTCTT164350.011.30646852
GCACACG186900.011.288794511
CGTCTGA190650.011.02984716
Produced by FastQC (version 0.11.5)